# Cys-containing

By [popomatter](https://paragraph.com/@popomatter) · 2021-10-28

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Each Cys-containing protein sequence in the NR database was initially searched against the _Olavius_ symbionts’ metagenomic database for possible TGA/TAG/TAA-containing homologs using TBLASTN with default parameters. Only local alignments, in which Cys in the query protein was aligned with TGA codon in the nucleotide sequence from the _Olavius_ symbionts’ metagenomic database, were selected for further analysis. For each TGA-containing nucleotide sequence identified in the metagenomic database, regions upstream and downstream of the putative in-frame TGA codon were analyzed to identify a minimal ORF. If a stop codon was found between the in-frame TGA codon and an initiation codon (ATG or GTG), such a TGA-containing sequence was discarded.

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*Originally published on [popomatter](https://paragraph.com/@popomatter/cys-containing)*