# PylT and PylS

By [Reddxdy](https://paragraph.com/@reddxdy) · 2021-10-28

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PylT and PylS sequences from _Methanosarcina barkeri_ (accession number [AY064401](https://www.ncbi.nlm.nih.gov/nuccore/AY064401)) were used to search for possible homologs in the metagenomic dataset. Candidate tRNAPyl was further analyzed to identify structural features associated with known tRNAPyl, including a six base-pair acceptor stem and a base between the D and acceptor stems . Other genes in the Pyl operon (_pylB_, _pylC_, _pylD_) were also analyzed by comparative sequence analyses.

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*Originally published on [Reddxdy](https://paragraph.com/@reddxdy/pylt-and-pyls)*