# PylT and PylS

**Published by:** [Reddxdy](https://paragraph.com/@reddxdy/)
**Published on:** 2021-10-28
**URL:** https://paragraph.com/@reddxdy/pylt-and-pyls

## Content

PylT and PylS sequences from Methanosarcina barkeri (accession number AY064401) were used to search for possible homologs in the metagenomic dataset. Candidate tRNAPyl was further analyzed to identify structural features associated with known tRNAPyl, including a six base-pair acceptor stem and a base between the D and acceptor stems . Other genes in the Pyl operon (pylB, pylC, pylD) were also analyzed by comparative sequence analyses.

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