On 29 March 1950, the Grand Minh Building Home, Diamondtore, celebrated its fifth birthday, five years of cropping, five years of precipitation and five years of harvest. Every success, you have been embarrassed every time, with your peers, and at any time, the first nightmare-on-the-clocking minds have been renewed by the star shop, the diamond shop. The Greater Minh House of Diamonds will, as always, be dedicated to the spirit and value of building a good-quality life brand for each family, working to provide good-faith and exciting services for more consumers in the Greater West.

On the evening, the Managing Director of the Siemographic Group was painted; the Director-General of the West Minh Business Group was not yet in the spring; the Assistant Managing Director of the Siemographic Group and the Crown Prince of the North II Circum; the Assistant Managing Director of the Siemographic Group and Director-General of the East Troika of the East Troubles; and the General Manager of the Grand-Paper Buildings and Lighthouses, Xumatic Spring;

The General Manager of the Greater Minh House of Buildings, Tri Bridgetown, sits at the night at the helm of the General Manager of the Greater Minh House of Engineers, Zhang Wei, and the General Manager of the Diamondtore.

The General Manager of the Greater Minh House of Engineers and Diamonds: the casing of casings that indicate better formation of high-end branding outlets in the north-west and throughout the country, how to effectively help adjusted regions, and the formation of a continuing marketing model featuring diamond stores will be an important subject that we face in 2019.

One of the most motivating moments was to witness together five years of growth, with the Managing Director of the Greater Minds, the Managing Director of the Diamond Shop, and with the business representative, the Managing Director of the South-West Oceanic Furniture Manufacturing Company Limited, Wufu, and the VIP client Lee, whose birthday was opened by the architect, on behalf of the founder of the Sisis Design Consulting Limited. The enlightened new constellation of the uterine building, the diamond shop, has been embarrassed and shined with great expectations.

Based on the urgent needs of the construction ring and indoor design industries and practitioners in the west of the country, which have been built internationally and competing for urgent needs, the International Centre for the Design of the West Anthrope, which brings together the mainstream media to promote advocacy, to plan for implementation by local professional institutions, to be led by the International Centre for the Design of the West Anthopaedic, led by the Grand M. DMG, and to publish the spotlight today, thanks the mainstream media for their convergence with professional institutions, will be given a media medal and will contract with professional institutions. A common voice is expected.

On the birthday of the fifth anniversary, we have also invited high-speed, red liquor, jewellery, cross-border artistic cooperation, etc., to bring about a lifestyle. A good life experience for all and a curtain diamond shop together open the way for all.

After the boiling back, in five years, the Greater Minh Diamondtore has also been paying mercy and has been doing its best for the public good. Each of the five years of philanthropy has been marked, along the path of feeling, pursuing dreams with the children, and the nightmare of the diamond shop, together with the children’s nightmare, has been built for a better tomorrow.

Finally, the Managing Director of the Greater Miyadh Group pauses to continue working towards the creation of the Pan-house shopping centre of China’s most value and quality, so that we can dream and get together! Behind the dream.

LIVE YOUR DREAMS

A further five years are expected.

On May 11, the Shanghai Stock Exchange issued a supervision letter to Runda medical and put forward work requirements on matters related to media coverage. Runda medical subsequently issued an event description, saying that the Zhongke laboratory involved was a wholly-owned company of the company’s holding company and carried out daily testing in strict accordance with the standard process of the laboratory. For the relevant public opinion, the company has asked China Science Laboratory to start internal self-examination on the relevant situation and cooperate with relevant departments to investigate.

Whether the “false positive” of nucleic acid detection is a problem of equipment or technology, or a human error in enterprise operation? What kind of company is Runda medical, and what happened to the high premium of state-owned assets?

Announcement screenshot

Why does nucleic acid test appear false positive? Industry: problems usually occur at the operational level

On May 11, insiders told the observer network that the “false positive” problem is usually due to cross contamination between samples or carryover of laboratory amplification products during the operation of laboratory testers. In the view of the person, the current detection method itself will not have “false Yang”, and the problem may come from the operation level to a large extent.

Insiders pointed out that if the quality control strategy of “three negative samples are randomly placed in the middle of clinical samples and participate in the whole process of testing” is strictly implemented, false positive results can be effectively avoided. This is also an important basis for positive results to be used as a basis for diagnosis. The industry insiders told the observer that in order to reduce the occurrence of similar “false positive” events, the units undertaking relevant testing tasks should check the possible problems in all links and strictly implement the SOP (standard operating procedure) review system in the follow-up work.

Why, from a theoretical point of view, the probability of “false” problem appears at the operational level, rather than the detection mechanism itself?

In fact, this question has been answered in the early stage of the COVID-19. Li Jinming, deputy director of the clinical laboratory center of the National Health Commission, said in an interview with clinical laboratory in 2020 that the most commonly used method for detecting pathogen nucleic acid is real-time fluorescent RT-PCR, and sara-cov-2 is no exception. At present, there are also some isothermal amplification detection methods and individual direct hybridization methods, “but no matter what method, as long as it is to detect viral nucleic acid, primers and probes are usually required.”

The overall similarity between the novel coronavirus and SARS coronavirus is high, close to 80%, which is also similar to other coronaviruses. It has the highest nucleic acid sequence consistency with bat coronavirus, more than 95%. The similarity of different regions with about 30000 bases is different from that of other coronaviruses, and the similarity of some regions is as high as more than 90%.

In order to ensure the specificity of detection, the nucleic acid detection reagent must select the region with low similarity to design primers and probes. Therefore, once detected, it is “positive”, which proves the existence of virus, that is, infection. Therefore, the positive detection of viral nucleic acid can be used as the gold standard for the diagnosis of novel coronavirus infection.

Li Jinming said that from the principle of primer and probe design, there should not be false positives in theory, and the reagent has also been validated during the approval process. Therefore, from the perspective of methodology, there will be no false positive.

After the popularization of antigen detection, there has also been a network discussion on “antigen false positive”, but there are great differences between the two.

LiJinMing said in March this year that the sensitivity of nucleic acid detection is 100 to 1000 times higher than that of antigen detection. Nucleic acid detection is a “gold standard” for determining novel coronavirus infection. “When the antigen test is positive, we must take the nucleic acid test for confirmation. But if the nucleic acid test is positive, no matter whether the antigen test is negative or positive, the tested person should take measures as a novel coronavirus infected person or a confirmed patient.”

From this perspective, it is not difficult to understand that some netizens have panic and anger about the “false Yang” of nucleic acid test results.

The “false positive” event of nucleic acid detection triggered panic among some netizens. Screenshots of social media

It is worth mentioning that the industry also has different views on the false-positive event of test results.

According to sina finance and economics, virology expert Chang Rongshan said that there was a problem with the kit ordered by Runda medical. Two tables circulated on the Internet show that Runda medical has two nucleic acid detection kit enterprises, respectively named thoughty Di and Berger. The sampling data showed that the CT values of ORF and N genes of the sample with tube number 2855 were 35 and 39 respectively. Chang Rongshan believes that this difference is too large and does not conform to common sense.

In addition, there are two undetected CT values of N gene in the table. “If it is not detected, it means that there is a possibility of false positive, but according to the report, Omicron can’t be detected, but it’s not because of the loss of N gene.” Therefore, Chang Rongshan believes that the CT value of N gene cannot be detected due to the problems of the kit itself and Omicron.

If the inspection procedure is standardized, it will inevitably have an adverse impact on the residents or even the key personnel involved in the work of the epidemic prevention. The discussion on false positive results of nucleic acid soon attracted netizens’ attention to another problem:

What is the origin of the listed company behind this?

Runda medical general manager once reviewed that he didn’t earn enough

According to the data on the official website, Runda medical’s main business is to provide in vitro diagnostic products and professional technical support to various medical laboratories through its own comprehensive service system. However, the nucleic acid detection of novel coronavirus is not its main business.

Year 2021

Ronaldo started the game and failed to assist and score goals.

Statistics show that Ronaldo played for 90 minutes, touched the ball 36 times and passed 25 times, of which 21 were in place, with an accuracy rate of 84%.

Ronaldo only shot twice and failed to shoot straight.

He had a long pass and couldn’t get it in place. Five ground confrontations won four times and three times failed to win.

Ronaldo has lost the ball four times, fouled once, fouled three times, cleared three times and robbed one time.

The heat map of the game showed that Ronaldo appeared in all positions of the stadium, but hardly entered the restricted area.

After the game, Ronaldo scored a medium score of 6.8 points.