Vik8848

Assembled

vik8848@newsletter.paragraph.com (Vik8848) — Thu, 28 Oct 2021 03:45:45 GMT

Assembled sequences of the Olavius symbionts’ metagenome were obtained from NCBI with the project accession number AASZ00000000 . The database contained 5597 genomic sequences, which corresponded to a total of 23.7 million nucleotides. Non-redundant (NR) protein database was downloaded from NCBI ftp server. This dataset contained a total of 4 644 764 protein sequences (1 603 127 260 amino acids). BLAST was also obtained from NCBI.

characterize

vik8848@newsletter.paragraph.com (Vik8848) — Thu, 28 Oct 2021 03:45:28 GMT

To characterize selenoproteome in these symbionts, we adopted a Sec/cysteine(Cys) homology-based search approach, which has been successfully used to characterize the selenoproteomes of both prokaryotes and one of the largest prokaryotic sequencing projects, the Sargasso Sea microbial sequencing project . We detected known selenoproteins present in this metagenomic dataset and identified several novel selenoproteins. Interestingly, one deltaproteobacterium, δ1 symbiont, contains at least 57 selenoproteins, which is the largest number of selenoproteins reported to date in any organism. In addition, several Pyl-containing proteins were identified and most were also found in the same δ1 symbiont. Our results provide new insights into understanding evolution and function of these rare amino acids.

Complex

vik8848@newsletter.paragraph.com (Vik8848) — Thu, 28 Oct 2021 03:45:07 GMT

Complex symbiotic relationships between bacteria and multicellular eukaryotes have evolved in several environments, but science has traditionally focused on interactions that are pathogenic . Recently, there has been increased recognition of symbiotic interactions that benefit both the microorganism and the host . A recent metagenomic analysis of the symbiotic microbial consortium of the marine oligochaete Olavius algarvensis, a worm lacking a mouth, gut and nephridia, revealed four major co-occurring symbionts, which belong to Deltaproteobacteria (δ1 and δ4) and Gammaproteobacteria (γ1 and γ3), as well as one minor Spirochaete species. Since some Deltaproteobacteria are selenoprotein-rich organisms , we analyzed the selenoproteomes of these symbionts to examine a possible relationship between selenium and symbiosis.