The remaining clusters were analyzed for occurrence of bacterial SECIS elements, located immediately downstream of the in-frame TGA codons, using bSECISearch program . The final clusters were manually analyzed and divided into three groups: known selenoproteins, new selenoproteins (clusters containing at least two different sequences with conserved in-frame TGA codons) and selenoprotein candidates (clusters containing only one sequence). It should be noted that sequencing errors that generate...

The remaining clusters were analyzed for occurrence of bacterial SECIS elements, located immediately downstream of the in-frame TGA codons, using bSECISearch program . The final clusters were manually analyzed and divided into three groups: known selenoproteins, new selenoproteins (clusters containing at least two different sequences with conserved in-frame TGA codons) and selenoprotein candidates (clusters containing only one sequence). It should be noted that sequencing errors that generate...

All clusters were automatically searched against NCBI NR and microbial databases using BLASTX and TBLASTX. Each predicted ORF containing an in-frame TGA was considered further only if at least two corresponding Cys-containing homologs were detected and the proportion of TGA/Cys pairs in the set of homologs was >50%.

All clusters were automatically searched against NCBI NR and microbial databases using BLASTX and TBLASTX. Each predicted ORF containing an in-frame TGA was considered further only if at least two corresponding Cys-containing homologs were detected and the proportion of TGA/Cys pairs in the set of homologs was >50%.